EAC(小鼠艾氏腹水癌细胞)悬浮细胞的分离方法
组织材料若来自血液、羊水、胸水或腹水的悬液材料，zui简单的方法是采用1000r/min的低速离心10分钟，若悬液量大，可适当延长离心时间，但速度不能太高，延时也不能太长，以避免挤压或机械损伤细胞，离心沉淀用无钙、镁PBS洗两次，用培养基洗一次后，调整适当细胞浓度后再分瓶培养，若选用悬液中某些细胞，常采用离心后的细胞分层液，因为，经离心后由于各种细胞的比重不同可在分层液中形成不同层，这样可根据需要收获目的细胞。不同比重的分层液的配制和具体分离方法详见淋巴细胞分离培养的章节。
细胞名称：EAC(小鼠艾氏腹水癌细胞)
组织来源：非小细胞肺癌细胞
培养条件：RPMI-1640+10%FBS
形态：贴壁；上皮细胞样
规格：500000cells/瓶
储存方法：液氮（冻存）或复苏培养。
运输方式：冻存的细胞干冰运输，复苏的细胞冰袋运输。
EAC(小鼠艾氏腹水癌细胞)是不断生长，分化的细胞群，因其经过遗传改造，致使其具有无限增长的潜能。体外生长的细胞系用于医疗或科研。实际上，连续细胞系可以用大量次培养基培养，随着代数的增加细胞的基因型和表型都有可能发生改变。我司经营各种细胞，品牌有ATCC、sciencell、ICLC细胞等，品质优秀，质量保证。公司保证细胞纯度在90%以上，同时经过微生物检测，保证不含有HIV、HBV、HCV、支原体、真菌及其他类型的细菌。
EAC(小鼠艾氏腹水癌细胞)：
kl1395 CEpiCM 角膜上皮细胞培养基 Corneal Epithelial Cell Medium 500 ml ATCC
kl1396 OEpiCM 卵巢上皮细胞培养基 Ovarian Epithelial Cell Medium 500 ml ATCC
kl1397 MSCM-sf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-serum free 500 ml ATCC
kl1398 MEpiCM 乳腺上皮细胞培养基 Mammary Epithelial Cell Medium 500 ml ATCC
kl1399 ATCC
kl1400 ATCC
kl1401 KM-acf 角质细胞培养基 Keratinocyte Medium-animal component free 500 ml ATCC
kl1402 FM-acf 成纤维细胞培养基 Fibroblast Medium-animal component free 500 ml ATCC
kl1403 STEMium-ACF 人类多能干细胞培养基-无动物组分 STEMium. Human Pluripotent Stem Cell Growth Medium-animal component
free 500ml ATCC
kl1404 MSCM-acf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-animal component free 500 ml ATCC
kl1405 ATCC
kl1406 ATCC
kl1407 MEM MEM MEM with Earle's Salts and L-Glutamine 500 ml ATCC
kl1408 MEM MEM MEM with Hank's Salts and L-Glutamine 500 ml ATCC
kl1409 MEM MEM MEM with Eagles's Salts and Glutamine 500 ml ATCC
kl1410 M199E M199E Medium 199 with Earle's Salts and L-Glutamine 500 ml ATCC
kl1411 M199H M199H Medium 199 with Hanks Salts and L-Glutamine 500 ml ATCC
kl1412 DMEM DMEM DMEM with L-Glutamine and Sodium Pyruvate 500 ml ATCC
kl1413 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 500 ml ATCC
kl1414 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 1L ATCC
kl1415 DMEM DMEM DMEM with L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml ATCC
kl1416 DMEM DMEM DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml ATCC
kl1417 DMEM-prf DMEM-prf DMEM with High Glucose - L-Glutamine and Sodium Pyruvate - phenol red-free 1L
ATCC
kl1418 Ham's F-10 Ham's F-10 F-10 with L-Glutamine and 25 mM HEPES 500 ml ATCC
kl1419 Ham's F-12 Ham's F-12 F-12 with L-Glutamine and 25 mM HEPES 500 ml ATCC
kl1420 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine 500 ml ATCC
kl1421 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine and 15 mM HEPES 500 ml ATCC
kl1422 RPMI 1640 RPMI 1640 RPMI 1640 without L-Glutamine - with 25 mM HEPES 500 ml ATCC
kl1423 RPMI 1640 RPMI 1640 RPMI 1640 with L-Glutamine and 25 mM HEPES 500 ml ATCC
kl1424 IMDM IMDM MDM with L-Glutamine and 25 mM HEPES; without alpha-Thioglycerol - 2-mercaptoethanol 500 ml ATCC
kl1425
kl1426
